Islam M El-Garawani and Zarraq I.A. Al-Faifi
Keywords: DNA, MN test, acrylamide, lemon grass, sage, protection
Abstract: Acrylamide (ACR) is commonly used in biochemistry and biotechnology laboratories. It may also occur in our daily life; in deep-fried foods; baked goods; tap water and smoking which is also a major ACR producer. In this work, the ameliorative role of lemon grass and sage (1:1) aqueous extract against the toxic potential of acrylamide was investigated after 48 hours of simultaneous treatments on albino rats. The 2, 2-diphenyl-1-picrylhydrazyl assay was carried out to evaluate the antioxidant potential of the plant mixture which gave more activity than each plant alone; perhaps due to synergistic effect between the two plants. The results of acridine orange/ethidium bromide fluorescent staining and total genomic DNA electrophoresis of peripheral blood leukocytes revealed that high dose of plant mixture treatments has a significant amelioration against ACR toxicity. On the other hand, low dose of plant mixture did not give that potential in micronucleus test of leukocytes, total genomic DNA electrophoresis of liver tissues and acridine orange/ethidium bromide fluorescent staining of bone marrow tissues which indicated that low dose was better as ameliorative agent
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