Madhulika Singh, VS Jaiswal and Uma Jaiswal
Keywords: Thidiazuron, shoot bud, peripheral zone, vascular tissue, safranin.
Abstract: Thidiazuron (TDZ) has potential for de novo multiple shoot induction without any callusing at any stage in Dendrocalamus strictus Nees. Longitudinal section through basal node region of the explants cultured in half strength Murashige and Skoog (MS) medium with 2.3?M TDZ showed shoot bud initials within a day of culture initiation while those cultured in TDZ free half strength MS medium did not show any evidence for the presence of shoot bud. The organization of shoot bud initial was tunica corpus type and in 3-4 days a short cylinder of provascular tissue is differentiated as the initial stage of vascular differentiation. After about 8 days of culture the newly differentiated bud began to elongate and new shoot bud initials were originated successively from actively dividing cells at the base of elongating shoots. The above process of regeneration continued even after transfer of explants into medium without TDZ.
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