GS George, AA Uwakwe and ET Egoro
Keywords: Reactive Glucose, dicarbonyls, glycated haemoglobin, hyperglycemic.
Abstract: To examine the effect of hyperglycemia on production of glucose by-products, we determined the concentration of glyoxal, methylglyoxal along with glucose, insulin and glycated haemoglobin (HbA1c). Using radioimmunoassay, chromatographic and spectophotometric techniques, the interactions of these compounds have been used to elucidate a possible cause of clinical significance in the diabetic state. Our study has shown that the selected analytes interacts in uncommon ways to exercerbate glycemic stress. We observed a positive linear relationship between glucose concentration and glycated haemoglobin and identified glucose excess as being a responsible factor controlling the creation of glucose by-products. Concomitant with the knowledge of the fact that both compounds are detoxified by the activity of their respective glyoxylase, we observed glyoxal concentration 3-4 folds higher than methylglyoxal in patients investigated. Further analysis of data with a scatter plot of subjects according to their blood glucose and HbA1c, glyoxal and methyloxal shows a positive linear correlation and the linear regression had a coefficient of r=0.820 significant at P > 0.05. The biochemical evidence suggests that production of carbonyls increases glucose toxicity and imposes the need to provide substances that will inhibit their formation. This is expected to enhance diabetes mellitus management.
[View Complete Article]