Iroha Ifeanyichukwu Romanus and Oji Anthonia Egwu
Keywords: Klebsiella pneumoniae, susceptibility, RAPD, clinical specimens
Abstract: We analyzed the antibiotic susceptibility profile of one hundred and fifty (150) Klebsiella pneumoniae strains isolated from different clinical samples (urine= 72, high vaginal swab=12, sputum=50 and wound swab= 16) isolated from patients visiting University of Nigeria teaching hospital (UNTH) Enugu, during June 2008 – May 2009. All samples were analyzed and organism isolated using standard Microbiology techniques, antibiotic susceptibility testing was carried out as described in the manual of antibiotic susceptibility testing. Clonal relatedness of all the K. pneumoniae strains was determined by randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). Antibiotic susceptibility studies revealed that K. pneumoniae from wound samples were the most susceptible strains followed by HVS, sputum and urine. The overall susceptibility profile are as follows; imipenem (100%), amikacin (100%), cefoxitin (99.4%), aztreonam (98%), ceftazidime (98%), cefotaxime (96.7%), amoxicillin/clavulanic acid (96%), ciprofloxacin (96%), tobramycin (93.3%), kanamycin (90%), cefuroxime (86.7%), gentamicin (76%), sulphamethoxazole/trimethoprim (22%), chloramphenicol (15.4%) and ampicillin (5%). RAPD analysis to determine the clonal relatedness of resistance strains grouped them into two clusters (A and B) based on band patterns. All strains resistance to ampicillin and chloramphenicol showed 100% similarity in band patterns (clonal group A) while strains resistance to sulphamethoxazole/trimethoprim showed different band patterns (clonal group B). Our study revealed strains of Klebsiella pneumoniae belonging to two clonal groups based on their resistant to ampicillin, chloramphenicol and sulphamethoxazole/trimethroprim.
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