Xing Yue (Larry) Pengand Paul CH Li
Keywords: Microfluidic device, centrifugal pumping, equiforce spiral channel, DNA microarray.
Abstract: In order to create a multi-sample-multi-probe DNA microarray on a circular disk (CD), we have designed numerous equiforce spiral microchannels on it. Hydrodynamic DNA sample hybridizations occurred in these channels when the samples were delivered by centrifugal pumping. The main goal of the use of the equiforce microchannel is to ensure a constant liquid flow velocity as the sample is filled through an empty spiral channel. Nevertheless, the design of the equiforce spiral channel has not been reported. In this work, the mathematical equation of the spiral curve and its physical basis for the equiforce condition are reported for the first time, and the constant flow velocity has been proven by a detailed image analysis of the liquid front advancing inside the microchannel. The sensitivity tests of various design parameters on the flow velocity in the spiral channel were revealed in detail.
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