DEVELOPMENT AND EVALUATION A SIMULTANEOUS ASSAY METHOD OF METRONIDAZOLE AND DICLOFENAC POTASSIUM IN A PHARMACEUTICAL FORMULATION Page No: 2333-2339

Nida’a MA Wadi, Khalid Shalaby, Peh Kok Khiang and Alka Ahuja

Keywords: Metronidazole (MET), diclofenac potassium (DIC-K),HPLC, validation.

Abstract: In this study, a simple, economic and selective HPLC method was developed and validated for the simultaneous estimation of Metronidazole (MET) and Diclofenac potassium (DIC-K). Reversed-Phase chromatography (RV-HPLC) was performed on a C18 column with methanol-Buffer 70:30%(V/V) as a mobile phase at a flow rate at 1ml/min. Metronidazole and Diclofenac Potassium have a maximum absorption at 254nm. The proposed method was successfully applied for determination of both drugs in one dosage form. Statistical analysis proved the method was precise, selective, specific and accurate for simultaneous analysis of Metronidazole and Diclofenac potassium.



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