Magda A El-Bendary , Samiha M Abo El-Ola and Maysa E. Moharam
Keywords: Lipase production, Poly(ethyleneterephthalate), surface modification, Bacillus, agro-industrial by-products.
Abstract: In this study, hydrolysis of the surface of poly(ethyleneterephthalate) (PET) fabric by Bacillus isolate 5W and 6C lipases was confirmed by improved hydrophilic property which tested by wetability and staining with basic dyes, scanning electron microscope, and FTIR measurements. Lipase treated PET fabrics showed good physical and mechanical properties. It was observed that the retained strength of lipase treated PET was 96-98% and pilling and static charges were reduced. Bacillus isolate 5W was chosen for economic production of PET surface modifying lipase enzyme in 14 agro-industrial by-products. The most suitable substrate for the highest lipase production was cotton seed meal at 6% and initial pH 7.5 (90U/ml). Effect of volume of the medium in the flask, inoculums size and incubation period were investigated and 25 ml of the medium, 25 x 106 CFU/ml, and 24h, respectively were the optimal. Addition of peptone to cotton seed meal medium enhanced the lipase production 22.5% (130.7 U/ml). The results of this article focuses on production of lipase enzyme that hydrolyze PET under feasible and economic conditions for replacing harsh chemical processes currently used for hydrophilization.
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