Zhongping Chen, Zhisheng Wang and Anguo Zhou
Keywords: Glucosamine, fungal protein, Aspergillus oryzae, solid state cultivation.
Abstract: The estimation of fungal protein is an essential parameter for nitrogen metabolism studies and evaluating the biological efficiency of fungal protein synthesis during fermented soybean meal processing. The aim of this study was to validate the fungal protein of Aspergillus oryzae SICC 3.302 measurement in solid state cultivation conditions using glucosamine content. Factors influencing the correlation between the glucosamine amount and fungal protein content, including incubation time, culture conditions (liquid or solid medium), carbon and nitrogen source, have been measured. Results suggest that the correlation of the glucosamine level and fungal protein content, regardless of incubation time and culture conditions, was a nearly constant. The influence of the medium composition, in particular the nitrogen source on the correlation has occurred. Collectively, these results indicated that glucosamine could be considered as reliable fungal protein indicator under the same nitrogen source.
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