Saber A. Sakr and Hany A. Abdel-Samie
Keywords: Ginko biloba, cyclophosphamide, rats, kidney, histology, histochemistry, immuno histochemistry, oxidative stress
Abstract: Cyclophosphamide (CP) is an anticancer drug utilized for treatment of many types of cancer. Botanical remedies became a promising tool for alleviation of side effects of many drugs. Among these natural plants is Ginko biloba (GB) that possesses therapeutic efficiency against many diseases. The present work studied the effect of GB extract on CP-induced nephrotoxicity in albino rats. Adult male rats were divided into 4 groups; control, GB-administered, CP-treated, and CP plus GB exposed groups. Treating rats with CP for 4 weeks caused renal veins congestion, intertubular leucocytic infiltrations, tubular epithelium degeneration and atrophied glomeruli. Treatment with CP+ GB showed an improvement in the structure of kidney cortex. Histochemical results showed that treatment with CP induced extreme decrease in total carbohydrates and proteins while treatment with CP and EGB restored these components in the majority of the cells. Immunohistochemically, an increase in ?-SMA expression was induced by CP whereas GB alleviated elevation of ?- SMA caused by CP. Serum creatinine, urea and lipid peroxidation marker, MDA were significantly elevated by CP treatment while there was a decrease in SOD and CAT. Treatment with CP+GB restored the normal values of these parameters. In conclusion, Ginko biloba extract exhibited protection against cyclophosphamide-induced renal toxicity in albino rats.
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