Nadia H Abd El-Nasser, Samia M Helmy, Amal M Ali, Abeer A Keera and Hala M Rifaat
Keywords: Antimicrobial substances, characterization, production, Streptomyces.
Abstract: Streptomyces viridodiastaticus isolated from Qalubiya soil of Egypt is capable to produce antibacterial and antifungal compounds. It showed the highest level of antimicrobial activity in the shaken culture broth after 7 days of incubation at neutral pH value using starch, potassium nitrate and dipotassium hydrogen phosphate amended medium. Two active fractions from the antimicrobial substance were extracted by diethyl ether at acidic pH and purified by Sephadex G-100 column chromatography. Elementary analysis indicated the absence of nitrogen from the two fractions. The empirical formulas for the two fractions (A) and (B) were C6H13O and C5H7O while the molecular formulas were C30H65O5 and C20H28O4 respectively. The ultraviolet, infrared and mass spectra of the purified substance (A) indicated the presence of hydrogen bond, methyl group, diketones, aliphatic compound, alkenes and hydroxyl group. The purified substance (B) contained all the groups present in substance (A) except hydroxyl group.
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