Hala M Rifaat and Osama Hamed El-Sayed
Keywords: Antimicrobial activity, decayed plants, rare actinomycetes, soil, water.
Abstract: Sixty-six of rare actinomycetes were isolated from samples of soil, fresh water and decayed plants collected from different Egyptian localities. They were recovered on humic-vitamen B agar medium using dilution, several antibiotics as selective agents and mild heat techniques. They assessed for their antimicrobial activity using diffusion assay methods against seven Gram-positive bacteria, seven Gram-negative bacteria, two yeasts and two filamentous fungi. Among the 66 isolates, 35 (53%) strains showed an activity against some of the test organisms. The polyphasic identification of the active isolates revealed that they are commonly Micromonospora (23 isolate, 65.71%), less commonly Actinoplanes (11 isolates, 31.43%) and rarely Dactylosporangium (1 strain, 2.86%) genera.
[View Complete Article]