BLUEBERRY ANTHOCYANINS ANALYZED BY ABSORPTION SPECTROSCOPY AND HPLC-UV-MS Page No: 765-772

Paul CH Li, Michael CK Wong, Hans Adomat and Emma S Tomlinson Guns

Keywords: anthocyanins, blueberry, visible absorption spectroscopy, liquid chromatography-mass spectrometry.

Abstract: Anthocyanins are found in various kinds of blueberry fruits. Since there are reported benefits of anthocyanins, there are numerous blueberry products available. We present a study of total anthocyanin quantification and individual anthocyanins examination of various blueberry samples. Total anthocyanins were found to be higher in two blueberry powder capsules (1.38±0.02 mg/g for WN, and 0.64±0.03 mg/g for WQ) than in blueberry juice (300±2 ?g/mL) using the pH differential method. Individual anthocyanins were characterised analytically using LC-UV-MS. Twenty-three anthocyanins were identified by HPLC-MS in 19 min. Interestingly, 4 new species were found and they were derived from two anthocyanidins: delphinidin and petunidin.



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